Dutch coach Louis van Gaal confirmed on the eve of the game in Sao Paulo that his star striker, who has scored three goals in Brazil, is a serious doubt for the last-four clash.
Asked if the 30-year-old will, play, Van Gaal said: "He has a stomach problem, an intestinal problem. I don't know.
"I will decide. Van Persie is our captain so he is very important to us. In principle my captain always plays, but he has to be fit."